| Transport of boar semen doses |
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For this purpose, we can use several preservation techniques: refrigeration at 15ºC, deep freezing and dialysis. In this chapter, we must take into account again the influence of temperature, light, air, time and extender depending on the technique applied. The temperature used to preserve the boar semen is a critical factor in order to get an optimal level of semen quality up to the insemination time. The rules that we must keep in order to transport boar semen at 15ºC are the same as in the last chapter: - It is advisable to avoid temperature changes during preservation and transport. At 15ºC, the metabolism of the sperm cell becomes slower and this allows the preservation of the sperm vitality for a longer period. - The upper and lasser limits of temperature that we must keep to a good preservation and recovery are respectively. On the other hand, allows the preservation of an ejaculate at -196ºC during an indefinite and to transport it into containers of liquid nitrogen to any place of the world. The action of light must be avoided also during transport, because it is a stimulating factor of the sperm metabolism. preservation and transport should be carried in anaerobic conditions, removing the air from the semen packing, thus preventing oxygen that also activates its metabolism. The period of time available to transport one semen dose depends on the technique used in its preservation. In case of cooling at 15ºC, the period of preservation depends to a great extent on the type of extender used (short term, up to 3 days and long term, up to 7 days or more). If we use the freezing technique, the period of time available to the semen transport becomes indefinitely longer. finally, the dialysis allows the preservation of a fresh ejaculate during several days. With this technique, the ejaculate is submitted to a process of ion and mineral salts exchange. The whole dialyzed ejaculate can be transported in a smaller space if it would be diluted. |