• LABORATORY: after the evaluation, the ejaculate is blended with the chosen extender that must be previously prepared at a temperature ± 1ºC regarding to ejaculate's. For this purpose, both extender and ejaculate must be placed into a water bath at 35ºC. Once the ejaculate has been diluted, it is left at room temperature (20-22ºC) for at least 2- 3 hours in order to achieve 20ºC gradually. Then it is placed into the fridge at 15ºC.

• PRESERVATION: in the 15ºC fridge the semen doses will remain a period of time that may vary depending on insemination demands of, ejaculate's quality and extender used. During all this period, temperature must not decrease below 15ºC. controlling it through  a maximum-minimum thermometer into the device.

• INSEMINATION: before insemination, semen doses are removed from the 15ºC fridge and are placed into a water bath at room temperature (20-22ºC). After 10-15 minutes, the water bath is switched on to get a final temperature of 35ºC along 10-15 minutes, so doses go from 15 to 35ºC at least during 20-30 minutes. Once at 35ºC, this temperature must be maintained without changes until the time of insemination. However, a long lasting period should be avoided since spermatozoa would become exhausted and die.

..It very important to remember that temperature decrease of to 15ºC must be gradual, because we submit the spermatozoa to a temperature against its physiology that provokes that plasma membrane becomes rigid and Dose heating can be faster  the spermatozoa go again to its physiological temperature.